Easily stain mycobacteria faster with Modified Auramine O.
The stain has been shown to have a specificity of 90%
Mycobacterial disease has been steadily increasing in the past 10 years. For public health reasons, it is vital to identify this disease as rapidly as possible. Traditional stains used are time-consuming and often difficult to read if decolorization is not performed properly. Auramine O and Auramine-Rhodamine stains have been used successfully but have 3 steps with a prolonged staining time. Our Modified Auramine O stain is faster and more efficient with just a 2 step process, omitting the permangante step.
Catalog Number |
Description |
Packaging |
|---|---|---|
| 345-250 | Modified Auramine O (stain & decolorizer / quencher) |
250ml bottle each / package |
| 345-01L | Modified Auramine O (stain & decolorizer / quencher) |
1 liter each / package |
| 345-04L | Modified Auramine O (stain & decolorizer / quencher) |
4×1 liter each / package |
The Scientific Device Laboratory Modified Auramine O Stain Set uses a modified stain and quenching/decolorizing agent to make a rapid two-step, 2 minute fluorescent staining procedure for the visualization of Mycobacteria organisms in clinical specimens.
Modified Auramine O Stain Set contains 2 stains:
- Modified Auramine O Stain (in either 250 ml or 1 gallon)
- Decolorizer/Quencher (in either 250 ml or 1 gallon)
Preparation of Patient Sputum Smear:
- Heat fix smear.
- Apply Auramine O Stain on smear for 1 minute.
- Rinse with deionized or tap water and drain slide.
- Apply Decolorizer/ Quencher reagent for 1 minute.
- Rinse with deionized or tap water and drain slide.
- Allow the slide to air dry.
- Read slide under mercury or halogen light source fluorescent microscope using recommended techniques.
View slides using a fluorescent microscope with a blue barrier filter (390-440 µ, blue) as used for Fluorocein isothiocyanate.
Mycobacteria fluoresce green against a dark background. All other organisms will not be visible. A fluorescent bacilli is the presumptive identification of Mycobacteria species. Cultures should be performed for confirmation. A negative result does not preclude the presence of Mycobacteria.
Prior to shipping each lot of the Modified Auramine O Stain Set has been quality controlled using as Acid Fast Control Slide. In addition, each lot should be quality controlled prior to use with standard laboratory procedures.
Safety:
- For In vitro diagnostic use. See MSDS for additional information.
- These reagents can be harmful or fatal to your health. Use them in a well-ventilated ara. Use proper gloves when staining. Keep away from flames. Good general microbiology techniques should be followed.
Storage:
- Store at room temperature (15°C to 30°C)
Warnings:
- Do not use product beyond the expiration date.
- Do not interchange or mix components from different staining sets.
- Cultures should be performed for confirmation on positive smears.
- A negative result does not preclude the presence of Mycobacteria.
- Avoid wiping slide dry with cloth or paper towels. Let slide air dry.
- A negative results
Some rapid growers do not fluoresce with this stain. Therefore, a Ziehl-Neelsen or Kinyoun stain should be performed on these specimens. The correct timing for stain and decolorizer should be observed. The turbidity of the stain will not affect the results. Smears should be viewed as soon as possible.
Watch the magic happen below.
John Hopkins and Loyola Medical Center concluded in two clinical studies that Modified Auramine O was more cost-effective and more efficient staining mycobacteria.
Organisms are so bright making them easier to see. With it being only a two-step process, omitting the permanganate step you can scan your smears faster.